Kanamycin Sulfate solution is an aminoglycoside antibiotic consisting of a six-membered aminocyclitol ring attached to aminosugar side chains through glycosidic bonds. Kanamycin Sulfate solution acts by inhibiting protein synthesis, and compromising translational fidelity in susceptible bacteria.
Description
Kanamycin Sulfate solution is an aminoglycoside antibiotic consisting of a six-membered aminocyclitol ring attached to aminosugar side chains through glycosidic bonds. Kanamycin Sulfate solution acts by inhibiting protein synthesis, and compromising translational fidelity in susceptible bacteria. Active against most strains of gram-negative bacteria, many strains of gram-positive bacteria and some strains of Mycobacterium. Often used as a transgenic marker in plants. Most active in alkaline solution.
Applications
Formulation: C18H36N4O11.H2SO4
Recommended Storage Condition: -20 °C
Linear acrylamide is widely used as one of the coprecipitants which are inert substances used to aid recovery of nucleic acids before alcohol precipitations.
TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C).
CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate ) is a zwitterionic detergent used to solubilize proteins. It is used as a non-denaturing solvent in the process of protein purification and is especially useful in purifying membrane proteins.
Ammonium Persulfate (APS) is an oxidizing agent that is used with TEMED to catalyze the polymerization of acrylamide and bisacrylamide to prepare polyacrylamide gels for electrophoresis.
25% Triton X-100 is a commonly used detergent in laboratories.
IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under the control of the lac operon.
Ammonium Sulfate. (NH4)2SO4 is an inorganic salt with a number of commercial uses.
Bovine Serum Albumin (BSA) has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions. MCLAB’s Ultrapure BSA is a "non-acetylated" BSA, pure enough to use when the integrity of DNA or RNA is essential. It has been tested for DNase, RNase, endonuclease, protease, peroxidase, and alkaline phosphatase activity, and assayed for fluorescence background.
Super-DITM Formamid jest zalecanym buforem obciążającym dla wszystkich sekwenatorów ABI.
Buffer EB is the elution buffer used in many Qiagen kits, e.g. PCR, Gel Extraction, Nucleotide Removal Kits, MinElute Kits and the QIAprep Miniprep Kits for DNA cleanup or small-scale plasmid purification. The purified DNA can also be eluted in TE (10 mM Tris-Cl, 1 mM EDTA, pH 8.0), but the EDTA may inhibit certain subsequent enzymatic reactions.
Low concentration solutions of DNA are prone to DNA degradation or other loss. It is critical that optimal methods are employed for DNA suspension and long-term storage.
EDTA, 0.5 M, pH 8.0, is a sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC).
5M solution of sodium chloride (NaCl) in high purity dH2O. Solution is 0.2 μm filtered and dispensed into durable, square Nalgene bottles.
TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis.
LB Agar plates contain 1.0% Tryptone, 0.5% yeast extract, 0.5% NaCl, 1.5% agar.
Kanamycin Sulfate solution is an aminoglycoside antibiotic consisting of a six-membered aminocyclitol ring attached to aminosugar side chains through glycosidic bonds. Kanamycin Sulfate solution acts by inhibiting protein synthesis, and compromising translational fidelity in susceptible bacteria.
Čeština
English
