Recombinant Protein A, Agarose
Recombinant Protein A is an immunoglobulin-binding protein derived from the cell wall of the bacterium Staphylococcus aureus.
This website uses cookies We use cookies to personalize content and ads, provide social media functions and analyze our traffic. We share information about how you use our site with our social media, advertising and analytics partners. Partners may combine this information with other information you have provided or obtained as a result of using their services.
Description:
Glutathione S-transferase (GST) gene fusion systems have been widely used for obtaining large amounts of desirable protein in Escherichia coli. The fusion protein, which contains a GST tail can then be purified through affinity chromatography. MCLAB’s glutathione agarose is designed for the specific purification of GST recombinant proteins and other glutathione-binding proteins. Glutathione agarose is uniquely formulated for excellent binding capacity and purity of the protein of interest.
Specifications
| Chromatography technique | GST-tagged protein purification |
| Matrix | Highly cross-linked 6% beaded agarose |
| Active group | Glutathione |
| Active group density | >40 umol/ml drained medium |
| Binding Capacity | > 25 mg horse liver GST/ml resin |
| Spacer | 1,4-bis(2,3-epoxypropoxy)butane (12 atom stable and uncharged ether hydrophilic linkage) |
| Bead geometry & size | Spherical 50 to 150 um |
| Bead mean diameter d50v | 90 um |
| pH Stability working range | 4 to 13 |
| pH Stability cleaning in place (cip) | 4 to 13 |
| Chemical stability | Stable to commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride, Stable in organic solvents, e.g. 50% methylformamide and 50% dioxane. |
| Storage | 2 to 8 °C in 20% Ethanol |
Recommended Storage Conditions: 2ºC to 8ºC.
Binding Buffer:
50 mM Tris
150 mM NaCl
pH 8.0
Elution Buffer:
50 mM Tris pH 7.8
0.15 M NaCl
1 mM EDTA
1 mM DTT
10 mM GST
Chwilowo nie możesz polubić tej opinii
Zgłoś komentarz
Zgłoszenie wysłane
Twoje zgłoszenie nie może zostać wysłane
Napisz swoją opinię
Recenzja została wysłana
Twoja recenzja nie może być wysłana
Recombinant Protein A is an immunoglobulin-binding protein derived from the cell wall of the bacterium Staphylococcus aureus.
SP Cross-linked Agarose Big Beads are strong ion exchangers designed for industrial applications. The large particle sizes (100-300 µm) and excellent physical stability of the base matrix ensure maintained speed even with viscous samples.
Glutathione S-transferase (GST) gene fusion systems have been widely used for obtaining large amounts of desirable protein in Escherichia coli.
Q Cross-linked Agarose High Performance has well-deserved reputations as highly successful anion and cation ion exchange media for purifying a wide range of biomolecules.
Epoxy-activated Agarose is a pre-activated affinity chromatography support for the immobilization of a variety of biomolecules that contain nucleophiles, such as hydroxyl, amino, or thiol groups. These groups couple to the epoxy groups on the support, which is then used for the purification of proteins, carbohydrates, or DNA.
Used in proteomics and protein chromatography, amino acid agarose is prepared by covalently coupling of amino acid through a 17 atom stable and uncharged ether hydrophilic linkage to highly cross-linked 6% agarose with bead mean diameter of 90 um.
Ni2+, Co2+, Cu2+, or Zn2+ charged nitrilotriacetic acid (NTA) coupled to Agarose CL-6B via a stable and uncharged long ether hydrophilic spacer arm, and offers high binding capacity and minimal non-specific binding.
EDTA, 0.5 M, pH 8.0, is a sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC).
TWEEN-40 is a class of emulsifiers used in some pharmaceutical and food preparations. It is often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquid derived from PEGylated sorbitan (a derivative of sorbitol) esterified with fatty acids.
Glutathione S-transferase (GST) gene fusion systems have been widely used for obtaining large amounts of desirable protein in Escherichia coli.
check_circle
check_circle