dsDNA Quantification Broad Range Kit (for microplate reader)

Description: 

BioDynami DNA/RNA quantification kits are designed for precise quantification of DNA samples across a wide range of concentrations using microplate readers.

The Quantification Broad Range Kit is developed for double-stranded DNA quantification and includes BR dye, BR dilution buffer, and two DNA standards. The assay is accurate for DNA concentrations ranging from 100 pg/µL to 1000 ng/µL and is highly selective for double-stranded DNA over RNA, as shown in the graph below.

Protocol:

The assay procedure is simple – just dilute the BR dye with the dilution buffer, add the DNA sample, then read the concentration using microplate reader.

Features:

Quantification kits have several advantages over traditional DNA quantification methods, such as high sensitivity, stability, and tolerance to contaminants.
The kit reduces analysis costs by 50% (compared to the catalog price of the dsDNA Quantitation Assay, Broad Range from Thermo Fisher Scientific).

DNA Selectivity and Sensitivity:
For testing, samples with and without RNA contamination were used in a series of input DNA amounts: 200, 400, 600, 800, and 1000 ng. The comparison results with the Thermo Fisher Scientific Quant-iT™ dsDNA Broad Range Assay Kit confirm the high accuracy and reliability of the BioDynami kit.

 Effect of Contaminants:

Common contaminants such as salts, free nucleotides, solvents, detergents, RNA, single-stranded DNA, or proteins are well tolerated in the assays.

Package Size:

• 1000 reactions (Cat. No. 40540B)

Quant-iT™ is a registered trademark of Thermo Fisher Scientific.