RNA Quantification High Sensitivity Kit (Half-Volume 100 µl Assay)

Quantification of RNA

For use with Qubit™ fluorometer

Linear range: 0.5-100 ng RNA

Wymagany jest adapter do probówek oraz probówki testowe o objętości 100 µL.

Firma BioDynami opracowała zestaw Qubit o połowie objętości 100 µL, co obniża koszty zestawu o 50% w porównaniu z zestawami 200 µL. Zestawy pozwalają zaoszczędzić 64% (standardowy zestaw 200 µL) oraz 80% (zestaw o połowie objętości 100 µL) kosztów w porównaniu z zestawami ThermoFisher. Dodatkowo, do przygotowania zestawów 100 µL można używać pipet wielokanałowych i stojaków na probówki 96-dołkowe, co skraca czas przygotowania 8–12 razy.

 

Effect of Contaminants:

The tests are well-tolerated by common contaminants such as salts, free nucleotides, solvents, detergents, RNA or protein, as shown in the table below.

Description: 

BioDynami DNA/RNA quantification kits are designed for accurate quantification of DNA and RNA samples across a wide concentration range using the Qubit™ fluorometer.

The RNA Quantification High Sensitivity Kit is developed for RNA quantification and includes HS Dye, HS Dilution Buffer, and two RNA standards. The assay is accurate for RNA concentrations ranging from 250 pg/µL to 100 ng/µL, as shown in the graph below. The RNA Quantification High Sensitivity Kit offers several advantages over traditional RNA quantification methods, such as stability, sensitivity, and tolerance to contaminants.

Protocol:

The test procedure is simple – just dilute the HS dye with the dilution buffer, add the RNA sample, and measure the concentration using the Qubit™ fluorometer.

Features:

  • Optimized for use with the Qubit™ fluorometer
  • Uses Qubit™ RNA High Sensitivity test settings
  • Reduces costs by 60%

The kit reduces analysis costs by 50% (compared to the catalog price of the Qubit RNA High Sensitivity product from Thermo Fisher Scientific).

DNA Selectivity and Sensitivity:

Comparison results with the Thermo Fisher Scientific kit confirm the high accuracy and reliability of the BioDynami kit.

 

 

Qubit™ is a registered trademark of Thermo Fisher Scientific.

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