dsDNA Quantification High Sensitivity Kit (for microplate reader)

Description: 

BioDynami DNA/RNA quantification kits are designed for precise quantification of DNA samples across a wide range of concentrations using microplate readers.

The dsDNA Quantification High Sensitivity Kit is specifically developed for the quantification of double-stranded DNA and includes HS dye, HS dilution buffer, and two DNA standards. The assay is accurate for DNA concentrations ranging from 10 pg/µL to 100 ng/µL and is highly selective for double-stranded DNA over RNA, as shown in the graph below.

Protocol:

The assay procedure is simple – just dilute the BR dye with the dilution buffer, add the DNA sample,  then read the concentration using microplate reader.

Features:

The Quantification Kits offer several advantages over traditional DNA quantification methods, such as high sensitivity, stability, and tolerance to contaminants.

The kit reduces analysis costs by 50% compared to the catalog price of the dsDNA Quantitation Assay, Broad Range from Thermo Fisher Scientific.

DNA Selectivity and Sensitivity:

For testing, samples with and without RNA contamination were used in a series of input DNA amounts: 20, 40, 60, 80, 100, and 120 ng. The comparison results with the Quant-iT™ dsDNA High Sensitivity Assay Kit from Thermo Fisher Scientific confirm the high accuracy and reliability of the BioDynami kit.

Effect of Contaminants:

The tests are well-tolerated by common contaminants such as salts, free nucleotides, solvents, detergents, RNA, single-stranded DNA, or protein, as shown in the table below.

Package Size:

• 1000 reactions (Cat. No. 40540H)

Quant-iT™ is a registered trademark of Thermo Fisher Scientific.