End Repair/dA-Tailing Master Mix

Description:

End Repair/dA-Tailing Master Mix is an optimized solution for the rapid preparation of DNA fragments for subsequent adapter ligation during next-generation sequencing (NGS) library construction. It combines three essential enzymatic reactions into a single step: end repair (blunting), 5′ phosphorylation, and 3′ dA-tailing. The result is DNA fragments with 5′ phosphorylated and 3′ dA-tailed ends, ready for adapter ligation.

The master mix format and its proprietary chemical composition simplify the reaction setup and streamline workflows. It offers a cost-effective choice for both small and large projects aiming for rapid processing and high-quality results. The mix ensures both sensitivity and efficiency by maximizing DNA yield for downstream ligation during library preparation.

Features:

• All-in-one step: Performs DNA end repair, 5′ phosphorylation, and 3′ dA-tailing in a single reaction
• High library conversion efficiency: Maximizes library yield for NGS prep
• Flexible input range: Works with 500 pg to 1 μg of fragmented DNA
• User-friendly format: Master mix greatly simplifies setup and workflows
• Minimal GC bias: Reduces bias across genomes with varying GC content

Applications:

• Preparation of DNA fragments for next-generation sequencing (NGS)
• Suitable for both small and high-throughput projects due to reaction efficiency and sensitivity
• Ideal for downstream adapter ligation using compatible products, such as BioDynami NGS Ligation Master Mix

Product sizes:

24 reactions (Cat. No. 40104S)
96 reactions (Cat. No. 40104L)