50% IGEPAL CA-630 (NP-40)

The loading dye increases the density of the sample and adds colours to the sample, thereby simplifying the loading process. The solution contains dyes that, in a electric field, move toward the anode at predictable rates.

Ammonium Persulfate (APS) is an oxidizing agent that is used with TEMED to catalyze the polymerization of acrylamide and bisacrylamide to prepare polyacrylamide gels for electrophoresis.

Ammonium acetate is a widely used reagent in molecular biology and chromatography. Suitable applications include the purification and precipitation of DNA and protein crystallization. Ammonium acetate is commonly used in HPLC and MS analysis of various compounds, such as oligosaccharides, proteins, and peptides.

DEPC-treated Water is deionized, high quality, molecular biology grade water.

IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under the control of the lac operon.

CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate ) is a zwitterionic detergent used to solubilize proteins. It is used as a non-denaturing solvent in the process of protein purification and is especially useful in purifying membrane proteins.

TWEEN-40 is a class of emulsifiers used in some pharmaceutical and food preparations. It is often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquid derived from PEGylated sorbitan (a derivative of sorbitol) esterified with fatty acids.

Kanamycin Sulfate solution is an aminoglycoside antibiotic consisting of a six-membered aminocyclitol ring attached to aminosugar side chains through glycosidic bonds. Kanamycin Sulfate solution acts by inhibiting protein synthesis, and compromising translational fidelity in susceptible bacteria.

EDTA, 0.5 M, pH 8.0, is a sterile-filtered solution of 0.5 M EDTA in H₂O treated with diethyl pyrocarbonate (DEPC).

Contains 100% active components and is a mixture of organic non - silicone polypropylene based polyether dispersions. Can be sterilized repeatedly.

Ammonium Sulfate. (NH4)₂SO₄ is an inorganic salt with a number of commercial uses.

HEPES Solution is a biological buffer useful in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture, and degree of chemical definition required for the cell culture application.

Acetylated BSA is used as a carrier protein and an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is vital. The acetylation process inactivates any trace of active nuclease activity. While the acetylation process does change BSA's binding characteristics and makes it inhibitory in PCR reactions, it is normally added to restriction digests.

Used to select for ampicillin resistance in mutated and transformed cells.

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range. TBS can shift pH relatively drastically as temperature changes, with the pKa declining approximately 0.03 units per degree Celsius rise in temperature.

TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis¹. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis.