MCLAB’s 7M Guanidine-HCl Solution is a ready-to-use solution of guanidine hydrochloride, which can be easily diluted and pH-adjusted to any concentration below 7M.
Description
MCLAB’s 7M Guanidine-HCl Solution is a ready-to-use solution of guanidine hydrochloride, which can be easily diluted and pH-adjusted to any concentration below 7M. In addition to increasing solubility of hydrophobic molecules, guanidine is a general protein denaturant, unfolding proteins and altering their structures. This can cause some proteins to be irreversibly altered upon interaction with guanidine solutions and may lose their binding function. Before any significant use of guanidine, it is best to experiment with a small amount of the sample and conclude whether the denaturing effects will adversely affect the intended use of the protein.
Applications
Solubilizing proteins from inclusion bodies
Increasing solubility of hydrophobic peptides and proteins
Denaturing proteins
Recommended Storage Condition
Store at room temperature
Caution: EXTREMELY CORROSIVE, HANDLE WITH CARE! Avoid ingestion and contact with skin.
TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C).
SDS, sodium dodecyl sulfate is an anionic surfactant. SDS is commonly used in laboratory as a component of buffer for cell lysis, cell lysis during DNA extraction, and mostly in SDS-PAGE running buffer. Indeed, SDS is an anionic detergent applied to protein samples to linearize proteins and to impart a negative charge to linearized proteins.
Linear acrylamide is widely used as one of the coprecipitants which are inert substances used to aid recovery of nucleic acids before alcohol precipitations.
CHAPSO (3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate) is a zwitterionic detergent used to solubilize proteins. Due to its low light absorbance in the ultraviolet region of the electromagnetic spectrum, CHAPSO is useful for researchers monitoring ongoing chemical reactions or protein-protein binding with UV/Vis spectroscopy.
LB Agar plates contain 1.0% Tryptone, 0.5% yeast extract, 0.5% NaCl, 1.5% agar.
MCLAB's high-purity 30% acrylamide/bis-acrylamide solution is a safer alternative to handling powdered acrylamide.
TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis.
Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range. TBS can shift pH relatively drastically as temperature changes, with the pKa declining approximately 0.03 units per degree Celsius rise in temperature.
Kanamycin Sulfate solution is an aminoglycoside antibiotic consisting of a six-membered aminocyclitol ring attached to aminosugar side chains through glycosidic bonds. Kanamycin Sulfate solution acts by inhibiting protein synthesis, and compromising translational fidelity in susceptible bacteria.
Ammonium Sulfate. (NH4)2SO4 is an inorganic salt with a number of commercial uses.
The β-galactosidase substrate X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic stain for β-gal activity, and is commonly used to distinguish between recombinants and nonrecombinants by lacZ α-complementation with appropriate vectors and hosts.
Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation.
Low concentration solutions of DNA are prone to DNA degradation or other loss. It is critical that optimal methods are employed for DNA suspension and long-term storage.
Ammonium Persulfate (APS) is an oxidizing agent that is used with TEMED to catalyze the polymerization of acrylamide and bisacrylamide to prepare polyacrylamide gels for electrophoresis.
Used to select for ampicillin resistance in mutated and transformed cells.
MCLAB’s 7M Guanidine-HCl Solution is a ready-to-use solution of guanidine hydrochloride, which can be easily diluted and pH-adjusted to any concentration below 7M.
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