Low concentration solutions of DNA are prone to DNA degradation or other loss. It is critical that optimal methods are employed for DNA suspension and long-term storage.
Description:
Low concentration solutions of DNA are prone to DNA degradation or other loss. It is critical that optimal methods are employed for DNA suspension and long-term storage. MCLAB's DNA Storage Buffer helps to preserve valuable DNA samples. It contains proprietary stabilizers and ingredients preserving high quality of DNA samples and preventing absorption of the target DNA to plastic tubes upon repeated freeze-thaw cycles. DNA Storage Buffer is suitable for long-term storage of small amounts of next generation sequencing (NGS) library , which would decrease the cluster numbers over time. It is also appropriate for making highly dilute NGS libraries during quantification assay.
It is recommended to handle MCLAB's DNA Storage Buffer with low adhesion/binding pipette tips and tubes for better performance.
Applications
Library long-term storage
Making highly dilute NGS libraries during quantification assay
Recommended Storage Condition: 4°C
Linear acrylamide is widely used as one of the coprecipitants which are inert substances used to aid recovery of nucleic acids before alcohol precipitations.
Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range. TBS can shift pH relatively drastically as temperature changes, with the pKa declining approximately 0.03 units per degree Celsius rise in temperature.
Ammonium Persulfate (APS) is an oxidizing agent that is used with TEMED to catalyze the polymerization of acrylamide and bisacrylamide to prepare polyacrylamide gels for electrophoresis.
TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C).
Bovine Serum Albumin (BSA) has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions. MCLAB’s Ultrapure BSA is a "non-acetylated" BSA, pure enough to use when the integrity of DNA or RNA is essential. It has been tested for DNase, RNase, endonuclease, protease, peroxidase, and alkaline phosphatase activity, and assayed for fluorescence background.
The β-galactosidase substrate X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic stain for β-gal activity, and is commonly used to distinguish between recombinants and nonrecombinants by lacZ α-complementation with appropriate vectors and hosts.
Contains 100% active components and is a mixture of organic non - silicone polypropylene based polyether dispersions. Can be sterilized repeatedly.
TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis.
Ammonium acetate is a widely used reagent in molecular biology and chromatography. Suitable applications include the purification and precipitation of DNA and protein crystallization. Ammonium acetate is commonly used in HPLC and MS analysis of various compounds, such as oligosaccharides, proteins, and peptides.
MCLAB's RNA Storage Buffer, with optimized reagents and pH value, provides better preservation and greater RNA stability for valuable RNA samples than the standard EDTA and TE Buffer or just water.
Reported use in all phases of detergent compounding and aqueous processing in the textile and paper industries, in industrial metal cleaners, acid cleaners, floor cleaners, detergent sanitizers and waterless hand cleaners.
TWEEN-40 is a class of emulsifiers used in some pharmaceutical and food preparations. It is often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquid derived from PEGylated sorbitan (a derivative of sorbitol) esterified with fatty acids.
The loading dye increases the density of the sample and adds colours to the sample, thereby simplifying the loading process. The solution contains dyes that, in a electric field, move toward the anode at predictable rates.
Reported use in all phases of detergent compounding and aqueous processing in the textile and paper industries, in industrial metal cleaners, acid cleaners, floor cleaners, detergent sanitizers and waterless hand cleaners.
Low concentration solutions of DNA are prone to DNA degradation or other loss. It is critical that optimal methods are employed for DNA suspension and long-term storage.
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