TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help to protect DNA and RNA.
Description
TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help to protect DNA and RNA.
Application
Tris–EDTA (TE) is routinely used for suspending nucleic acid samples.
Physical form
10 mM Tris-HCl; 1 mM EDTA
Recommended Storage Condition: room temperature
The loading dye increases the density of the sample and adds colours to the sample, thereby simplifying the loading process. The solution contains dyes that, in a electric field, move toward the anode at predictable rates.
TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C).
A routine use of agarose is ideal for daily analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern) and is also suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID). It has low ethidium bromide and SYBR Green background staining.
DEPC-treated Water is deionized, high quality, molecular biology grade water.
MCLAB’s 7M Guanidine-HCl Solution is a ready-to-use solution of guanidine hydrochloride, which can be easily diluted and pH-adjusted to any concentration below 7M.
Brij-35 (30% Solution) is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or as a surfactant in various HPLC applications.
Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range. TBS can shift pH relatively drastically as temperature changes, with the pKa declining approximately 0.03 units per degree Celsius rise in temperature.
Ammonium Persulfate (APS) is an oxidizing agent that is used with TEMED to catalyze the polymerization of acrylamide and bisacrylamide to prepare polyacrylamide gels for electrophoresis.
EDTA, 0.5 M, pH 8.0, is a sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC).
TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis.
TWEEN 20 helps to prevent non-specific antibody binding in enzyme immunoassay as a washing agent (e.g. Western blots and ELISAs). In this major application, it is dissolved in Tris-Buffered Saline or Phosphate buffered saline at dilutions of 0.05% to 0.5% v/v.
5M solution of sodium chloride (NaCl) in high purity dH2O. Solution is 0.2 μm filtered and dispensed into durable, square Nalgene bottles.
Bovine Serum Albumin (BSA) has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions. MCLAB’s Ultrapure BSA is a "non-acetylated" BSA, pure enough to use when the integrity of DNA or RNA is essential. It has been tested for DNase, RNase, endonuclease, protease, peroxidase, and alkaline phosphatase activity, and assayed for fluorescence background.
Linear acrylamide is widely used as one of the coprecipitants which are inert substances used to aid recovery of nucleic acids before alcohol precipitations.
Used to select for ampicillin resistance in mutated and transformed cells.
Contains 100% active components and is a mixture of organic non - silicone polypropylene based polyether dispersions. Can be sterilized repeatedly.
TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help to protect DNA and RNA.
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