Description
TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help to protect DNA and RNA.
Application
Tris–EDTA (TE) is routinely used for suspending nucleic acid samples.
Physical form
10 mM Tris-HCl; 1 mM EDTA
Recommended Storage Condition: room temperature
HEPES Solution is a biological buffer useful in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture, and degree of chemical definition required for the cell culture application.
Bovine Serum Albumin (BSA) has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions. MCLAB’s Ultrapure BSA is a "non-acetylated" BSA, pure enough to use when the integrity of DNA or RNA is essential. It has been tested for DNase, RNase, endonuclease, protease, peroxidase, and alkaline phosphatase activity, and assayed for fluorescence background.
A routine use of agarose is ideal for daily analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern) and is also suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID). It has low ethidium bromide and SYBR Green background staining.
Ammonium Persulfate (APS) is an oxidizing agent that is used with TEMED to catalyze the polymerization of acrylamide and bisacrylamide to prepare polyacrylamide gels for electrophoresis.
TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis.
Low concentration solutions of DNA are prone to DNA degradation or other loss. It is critical that optimal methods are employed for DNA suspension and long-term storage.
SDS, sodium dodecyl sulfate is an anionic surfactant. SDS is commonly used in laboratory as a component of buffer for cell lysis, cell lysis during DNA extraction, and mostly in SDS-PAGE running buffer. Indeed, SDS is an anionic detergent applied to protein samples to linearize proteins and to impart a negative charge to linearized proteins.
Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range. TBS can shift pH relatively drastically as temperature changes, with the pKa declining approximately 0.03 units per degree Celsius rise in temperature.
D-(+)-Glucose is a common natural sugar involved in processes such as energy production, glycosylation, and formation of glycans that provide structure to cells. Glucose is involved in a detrimental process in cells called glycation. Glucose is used as a supplement for cell culture and in numerous cellular processes and molecular biology applications.
MCLAB's RNA Storage Buffer, with optimized reagents and pH value, provides better preservation and greater RNA stability for valuable RNA samples than the standard EDTA and TE Buffer or just water.
CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate ) is a zwitterionic detergent used to solubilize proteins. It is used as a non-denaturing solvent in the process of protein purification and is especially useful in purifying membrane proteins.
5M solution of sodium chloride (NaCl) in high purity dH2O. Solution is 0.2 μm filtered and dispensed into durable, square Nalgene bottles.
Contains 100% active components and is a mixture of organic non - silicone polypropylene based polyether dispersions. Can be sterilized repeatedly.
TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help to protect DNA and RNA.
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