Exonuclease I

Description:
Exonuclease I cleaves single-stranded DNA in the 3' -> 5' direction, releasing 5'-mono/di-nucleotides and leaving double-stranded DNA molecules and the 5'-terminus intact. The enzyme is processed through digestion and is inhibited by the presence of a 3'-terminal phosphate. Exonuclease I is tolerant of a wide-range of buffer conditions and can typically be added to reactions containing magnesium.

Specific Activity: 185,000 U/mg

Application:
- Removal of residual ssDNA, including oligos, from reaction mixes

Source:
Purified from a strain of E. coli that expresses the recombinant Exonuclease I gene.

Supplied in:
10 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.5 mM EDTA
50% Glycerol
pH 7.5 @ 25°C
 

10x Exonuclease I Buffer
670 mM Glycine-KOH
67 mM MgCl₂
100 mM 2-mercaptoethanol
(pH9.5@25°C)

Unit Definition:
One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble total nucleotide in 30 minutes at 37°C.

Recommended Storage Condition: -20°C