Description:
Alkaline Phosphatase SAP1 catalyzes the hydrolysis of phosphate monoesters. Has higher turnover rate and milder conditions of inactivation than bacterial enzyme. Can be used to remove 5′-phosphates from DNA or RNA prior to 5′-end labeling. Used to remove 5′-phosphates from linearized vector molecules to prevent self-ligation of the vector during cloning procedures. Applicable to dephosphorylation of proteins.
Storage Buffer:
10 mM Tris-HCl (pH 8.0 at 22°C)
50 mM KCl
1 mM MgCl2
0.1 mM ZnCl2
50% (v/v) glycerol
Unit Definition:
One unit is the amount of enzyme required to hydrolyze 1 μmol of p-nitrophenylphosphate to p-nitrophenol in 1 min at 37oC in a buffer of 1 M diethanolamine, 10 mM p-nitrophenylophosphate, 0.25 mM MgCl2 (pH 9.8).
Recommended Storage Condition: -20°C
Cost savings
Easy integration
High performance
The EX Terminator™ Purification Kit is intended for removal of unincorporated dye terminators and other inpurities.
Cost savings
Easy integration
High performance
Fast and simple to use
Minimal DNA loss
Direct use of the product
GMC-SAP01 - 500 U
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