BRAF V600 Mutations Detection Kit

Description:

AmoyDx^® BRAF V600 Mutations Detection Kit is an in vitro real-time PCR assay. The kit enables qualitative detection of V600 mutations in BRAF gene  in human DNA extracted from formalin-fixed paraffin-ded (FFPE) tissue samples. The kit is for in vitro diagnostic use and is intended for use by trained professionals in a laboratory setting.

Principle:

The kit adopts Amplification Refractory Mutation System (ARMS) and real-time PCR technology, which comprises specific primers and fluorescent probes to detect BRAF V600 mutations in human genomic DNA. During the nucleic acid amplification, the targeted mutant DNA is matched with the bases at 3’ end of the primer, amplified selectively and efficiently, then the mutant amplicon is detected by fluorescent probes labeled with FAM. While the wild-type DNA cannot be matched with specific primers, there is no amplification occurs.

High sensitivity: up to 1%
 

Detected mutations:

V600E, V600K, V600E2, V600R, V600D, V600D2

Tests/kit: 24 (bulk)

Instrument: Stratagene Mx3000P™, ABI 7500, ABI 7900HT, ABI StepOnePlus, LightCycler480 II, Bio-Rad CFX96, SLAN-96S