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MC Cloning 5’/3’-RACE Kit
The MC Cloning 5'/3' RACE Kit provides a convenient method for performing both 5' and 3' rapid amplifications of cDNA ends (RACE) with optimized primers for cloning any gene of interest.
Description:
First Strand cDNA Synthesis Kit can be used to synthesize the first strand cDNA by reverse transcription (RT) at higher temperatures than the wild type M-MuLV to reach higher cDNA yields for difficult RNA transcriptions. This kit uses MCLAB's QuantumScript™ HD Reverse Transcriptase, a unique mutation with increased thermostability and reduced RNase H activity.
Applications:
cDNA Synthesis
RT-PCR
RT-qPCR
Components:
|
Components |
Cap Color |
Volume (50 rxn) |
Volume (250 rxn) |
|
5X RT Buffer |
Red |
125 μl |
625 μl |
|
RT Enzyme Mix |
Clear |
100 μl |
500 μl |
|
DTT |
Yellow |
50 μl |
250 μl |
|
dNTP |
Blue |
50 μl |
250 μl |
|
Oligo dT Primer |
Green |
50 μl |
250 μl |
|
Random Primer |
Purple |
50 μl |
250 μl |
Storage Condition:
-20 °C. It is recommended to reduce freeze-thaw cycles as few as possible.
Shipping Condition: Dry ice
FSCS-100
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