Čeština
Polski
dsDNA Quantification High Sensitivity Kit
Quantification of dsDNA
For use with Qubit™ fluorometer
Linear range: 0.1 - 120 ng dsDNA
Description:
BioDynami DNA/RNA quantification kits are designed for accurate quantification of DNA and RNA samples across a wide concentration range using the Qubit™ fluorometer.
The Short Oligo Quantification Kit is developed for quantification of oligos shorter than 50 nt and includes dye, dilution buffer, and two standards. The assay is accurate for oligo concentrations ranging from 50 pg/µL to 200 ng/µL based on comparison of the data with standards, as shown in the graph below.
Protocol:
The test procedure is simple – just dilute the dye with the dilution buffer, add the oligo sample (volume 1–20 μL), and then measure the oligo concentration using the Qubit® fluorometer.
Features:
- Quantify the concentration of oligos from 11 to 50 nt
- Uses the Qubit™ ssDNA assay setting
- Linear range: 1–200 ng oligo
Quantification:
Long oligos (>50 nt) and ssDNA can be quantified using ssDNA Quantification kits. However, quantifying the concentration of oligos shorter than 50 nt is challenging due to data inaccuracy. Therefore, the Short Oligo Kit was developed, which is the most accurate for quantifying short oligonucleotides, especially for very short sequences (11 nt), where other kits fail. A comparison of the kits can be seen in the graph below.
Effect of Contaminants:
The tests are well-tolerated by common contaminants such as salt, solvents and detergents as shown in the table below.
Package Size:
- 100 reactions (cat. no. 40046S)
- 500 reactions (cat. no. 40046L)
Qubit™ is a registered trademark of Thermo Fisher Scientific.
40046S
New
Comments (0)
No customer reviews for the moment.
14 other products in the same category:
dsDNA Quantification Broad Range Kit (Half-Volume 100 µl Assay)
Quantification of dsDNA
For use with Qubit™ fluorometer
Linear range: 4-2000 ng dsDNA
Assay Tubes (Qubit™ Fluorometer) for half-volume 100 µl assays
High quality
Cost savings
Designed for Qubit™ fluorometer
ssDNA Quantification Kit (Half-Volume 100 µl Assay)
Quantification of ssDNA
For use with Qubit™ fluorometer
Linear range: 1-200 ng ssDNA
dsDNA Quantification High Sensitivity Kit (for microplate reader)
Quantification of dsDNA
Designed for a microplate reader
Linear range: 0.2-100 ng dsDNA
dsDNA Quantification Broad Range Kit (for microplate reader)
Quantification of dsDNA
Designed for microplate readers
Linear range: 4-1000 ng dsDNA
ssDNA Quantification Kit
Quantification of ssDNA
For use with Qubit™ fluorometer
Linear range: 1-200 ng ssDNA
dsDNA Quantification High Sensitivity Kit (Half-Volume 100 µl Assay)
Quantification of dsDNA
For use with Qubit™ fluorometer
Linear range: 0.1 - 120 ng dsDNA
RNA Quantification High Sensitivity Kit (Half-Volume 100 µl Assay)
Quantification of RNA
For use with Qubit™ fluorometer
Linear range: 0.5-100 ng RNA
dsDNA Quantification Broad Range Kit
Quantification of dsDNA
For use with Qubit™ fluorometer
Linear range: 4-2000 ng dsDNA
RNA Quantification High Sensitivity Kit
Quantification of RNA
For use with Qubit™ fluorometer
Linear range: 0.5-100 ng RNA
Viewed products
Quantification of oligo < 50 nt
For use with Qubit™ fluorometer
Linear range: 1-200 ng oligo