Recombinant Human NGF R100W Mutant

Description
Nerve Growth Factor (NGF) holds great therapeutic promise for Alzheimer's disease, diabetic neuropathies, ophthalmic diseases, and dermatological ulcers. However, the necessity for systemic delivery has hampered the clinical applications of NGF due to its potent pro-nociceptive action. A "painless" human NGF (hNGF R100W) mutant has been engineered. It has equal neurotrophic potency to hNGF but a lower nociceptive activity.

Full Name
Human NGF R100W Mutant

Source
E. coli

Species
Human

Accession #
CAA36832

Molecule Weight
The 120 amino acid recombinant protein has a predicted molecular mass of approximately 13.5 kD as monomer.

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method

Applications
ELISA, Functional Assay

Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is typically 0.2-2 ng/ml.

Storage
-80°C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS