HoTaq™ DNA Polymerase

Description:
HoTaq™ DNA Polymerase is a hot-start Taq DNA polymerase that is a chemically modified form of conventional Taq DNA polymerase. The enzyme remains inactive at room temperature and becomes active only after a 10-minute incubation at 95 °C, preventing non-specific amplification and primer-dimer formation during PCR setup. Its high specificity makes it suitable for routine and diagnostic PCR applications. The enzyme exhibits 5'→3' DNA polymerase activity but lacks 3'→5' exonuclease (proofreading) activity.

Features:

• hot-start Taq DNA polymerase
• activated after 10 minutes at 95 °C
• high PCR specificity
• minimizes non-specific amplification and primer-dimers
• 5'→3' DNA polymerase activity
• no 3'→5' exonuclease (proofreading) activity
• supplied with 10× Taq PCR Buffer (without dNTPs)

Source:

• recombinant Taq DNA polymerase expressed in Escherichia coli
• chemically modified Hot-Start technology

Unit Definition:
One unit (U) is defined as the amount of enzyme that incorporates 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 75 °C.

Applications:

• routine PCR
• diagnostic PCR
• DNA amplification
• molecular biology workflows
• laboratory research