Exo-Resistant Random Hexamer

Description:
Exo-Resistant Random Hexamer is a mixture of single-stranded random oligonucleotides designed for highly efficient random priming of DNA synthesis. Each primer contains two 3’-terminal phosphorothioate (PTO) modifications that protect against degradation by 3’→5’ exonuclease activity of proofreading DNA polymerases such as Klenow Fragment and phi29 DNA polymerase. The product has 5’ and 3’ hydroxyl ends and is supplied as a ready-to-use 20× concentrated solution.

Features:

• mixture of random hexamer primers
• 3’ phosphorothioate modifications
• resistant to proofreading exonuclease activity
• 5’ and 3’ hydroxyl ends
• 20× concentrated solution
• ready-to-use format

Applications:

• strand displacement amplification of genomic DNA
• plasmid and phage DNA amplification
• DNA labeling by random priming
• primer extension reactions
• cDNA synthesis and genomic workflows

Storage:

• store at −20 °C