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Source: Sphaerotilus species
5’-A C T A G T-3’
3’-T G A T C A-5’
Reaction Temperature: 37°C
Prototype: SpeI
Inactivation Temperature (20 min): 65°C
Reaction Buffer:
1x ONE Buffer
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Ad-2 DNA in 1 hr in a total reaction volume of 50 µl.
Storage Conditions: Store at –20°C.
Quality Control:
All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities.
E2398-01
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