ssDNA Quantification Kit

ssDNA Quantification Kit

Quantification of ssDNA

For use with Qubit™ fluorometer

Linear range: 1-200 ng ssDNA

Description: 

BioDynami DNA/RNA quantification kits are designed for accurate quantification of DNA and RNA samples across a wide concentration range using the Qubit™ fluorometer.

The ssDNA Quantification Kit is developed for quantification of single-stranded DNA and includes dye, dilution buffer, and two ssDNA standards. The assay is accurate for DNA concentrations ranging from 50 pg/µL to 200 ng/µL, based on comparison of the data with standards, as shown in the graph below.

The kit detects ssDNA using a fluorescent dye, which enables sensitive quantification of single-stranded DNA, including ssDNA viruses, synthetic ssDNA, the first stage of cDNA synthesis, denatured DNA, and bisulfite-converted DNA, etc.

Protocol:

The test procedure is simple – just dilute the ssDNA dye with the ssDNA dilution buffer, add the DNA sample (volume 1-20 μL), and then measure the concentration using the Qubit™ fluorometer.

Features:

  • Optimized for use with the Qubit™ fluorometer
  • Uses the Qubit™ ssDNA assay setting
  • Linear range: 1–200 ng dsDNA
  • Cost-effective – reduces costs by 50%
 

The kit reduces analysis costs by nearly 50% (compared to the catalog price of the Qubit ssDNA product from Thermo Fisher Scientific).

DNA Selectivity and Sensitivity:

For testing, samples with and without RNA contamination were used in a series of input DNA. The comparison results with the kit from Thermo Fisher Scientific confirm the high accuracy and reliability of the BioDynami kit.

Effect of Contaminants:

The tests are well-tolerated by common contaminants such as salt, solvents and detergents as shown in the table below.

 

Qubit™ is a registered trademark of Thermo Fisher Scientific.

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