APE1

Description:
APE1, also known as HAP1 or Ref1, acts as an AP lyase by hydrolyzing the phosphodiester backbone at the 5′-end of an apurinic (AP) site, generating a 1 base gap in the DNA duplex and leaving 3′-hydroxyl and 5′-deoxyribose phosphate termini. Evidence suggests that APE1 may exhibit weak DNA 3′-diesterase, 3′ to 5′-exonuclease and RNase H activities.

The specific activity is 2,000,000 U/mg. The concentration of the supplied enzyme is 15,000 U/ml.

The package includes a 10X green buffer (200 mM Tris-acetate; 500 mM potassium acetate; 10 mM dithiothreitol; 100 mM magnesium acetate, pH 7.9 at 25°C).

Applications:

• Single cell gel electrophoresis (Comet assay)
• Alkaline elution
• Alkaline unwinding
• Modified nick translation
• Source:
  An E. coli strain which carries the cloned human APE1 gene.
• Storage:
• -20°C
• Size:
  3,750 units (catalog no. APE-100)
  15,000 units (catalog no. APE-200)
  (Unit definition: One unit is defined as the amount of enzyme required to cleave 20 pmol of a 34-mer oligonucleotide duplex containing a single AP site in 1 hour at 37°C.)