Description:
FEN1 (AFU) is a recombinant flap endonuclease-1 (FEN-1) protein from the E. coli containing FEN1 Gene of hyperthermophilic Archaea strain,Archaeoglobus fulgidus. The FEN1 removes 5'-overhanging flaps in DNA repair and processes the 5'-ends of Okazaki fragments in lagging strand DNA synthesis.
Application:
FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis.
Source: E coli
Concentration: 0.5 mg/ml
Supplied In:
50 mM Tris-HCl (pH 8.0)
50 mM NaCl
1 mM DTT
0.1 mg/ml BSA
50% (v/v) glycerol
Supplied With:
10x Taq PCR Buffer (no dNTP)
Recommended Storage Condition: -20°C
Fast and simple fragmentation
Fragment size easily controlled by incubation time
Ideal for NGS library preparation
Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. CasRx, Cas13d from Ruminococcus flavefaciens XPD3002, is highly active and short, 94KD.
origin: E. coli carrying the Tn5 gene
random transposon insertion into DNA
requires 19-bp ME sequence
Fast and simple fragmentation
Fragment size easily controlled by incubation time
Ideal for NGS library preparation
Benzonase endonuclease from Serratia marcescens, can be used to degrade all forms of DNA and RNA while having no proteolytic activity.
Fast and simple fragmentation
Fragment size easily controlled by incubation time
Ideal for NGS library preparation
Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. CasRx, Cas13d from Ruminococcus flavefaciens XPD3002, is highly active and short, 94KD.
Cas9 Nuclease, Streptococcus pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA.
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