2X qPCR Master Mix (Probe-Based)

Description:

2X qPCR Master Mix (Probe-Based) is a ready-to-use reaction mixture designed for real-time PCR using fluorescent probes such as TaqMan or other sequence-specific probes. The mix contains Taq DNA polymerase, dNTPs, MgCl₂, and an optimized buffer, all prepared for immediate use – simply add primers, probe, and DNA template.

Amplification curves confirm the high sensitivity and efficiency of the 2X qPCR Master Mix (Probe-Based). Samples reach detectable signal levels at the expected cycle, indicating reliable and reproducible amplification without inhibition or significant delay. The product is therefore suitable for accurate real-time DNA quantification.

Features:

• Ready-to-use mix for real-time PCR with fluorescent probes (e.g., TaqMan)
• Contains Taq DNA polymerase with 5’→3’ exonuclease activity for probe cleavage
• Optimized buffer for high sensitivity, specificity, and robust performance
• Compatible with most real-time thermal cyclers
• Includes passive ROX dye for well-to-well normalization and instrument compatibility
• Reduces contamination risk due to fewer pipetting steps
• Suitable for a wide range of applications

Applications:

• Gene expression quantification

• Genotyping

• Pathogen detection

• High-throughput analysis

• Post-amplification melting curve analysis

Package sizes:

• 100 reactions (Cat. No. 50502PS)
• 500 reactions (Cat. No. 50502PL)