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Description:
Ribonuclease III (Rnase III) is an endoribunuclease that cleaves double-stranded RNA, resulting in 12-15 bp dsRNA fragments with 2-base, 3’-overhangs, and 5' phosphate and 3' hydroxyl termini.
Source:
Ribonuclease III (RNase III) from E. coli is an endoribonuclease that specifically digests dsRNA to dsRNA fragments that have two-base, 3' overhangs.
Application:
- Digestion of dsRNA to short fragments
- RNA structure, processing, and maturation studies
Unit Definition:
1 unit of the Rnase III digests 1 µg of dsRNA in 20 minutes at 37°C
Supplied With:
10X RNase III Reaction Buffer
10X RNase III Reaction Buffer:
500 mM NaCl
100 mM Tris pH 7.9
100 mM MgCl2
10 mM DTT
Storage Buffer:
Supplied in 50% Glycerol, 50 mM Tris-HCl (pH 7.5),100 mM NaCl, 0.1 mM EDTA.
Recommended Storage Condition: -20°C
RN3E-100
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