RNase H, Tth

RNase H, Tth

RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules.

Description
Thermostable RNase H specifically degrades the RNA in a DNA:RNA hybrid, without affecting DNA or unhybridized RNA. This thermostable nuclease exhibits the same enzymatic properties as E. coli RNase H, but is active at much higher temperatures. It has optimal activity above 65°C and can be used at temperatures up to 95°C. The thermostability of the enzyme permits it to be used at temperatures that give the highest hybridization stringency for specific DNA:RNA heteroduplexes, maximizing sensitivity and selectivity while minimizing background due to nonspecific hybridization.


Applications

  • Removal of poly(A) tails of mRNA hybridized to poly(dT)
  • Removal of mRNA during second strand cDNA synthesis


Source:
A recombinant E. coli strain carrying the RNAse H gene from the thermophilic eubacterium Thermus thermophilus HB8.

Storage Buffer:

50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1.0 mM DTT, 0.1 mM EDTA, and 0.1% Triton® X-100 and 50% glycerol.

10x RNAse H Buffer:
500 mM Tris-HCl, 750 mM KCl, 30 mM MgCl2, 100 mM DTT, pH 8.3 @ 25°C


Unit Definition:
One unit results in the acid-solubilization of 1 nmole of [3H]-polyadenylic
acid in the presence of an equimolar concentration of polythymidylic acid in 20 minutes at 45°C.

Recommended Storage Condition: -20°C

RNHT-100
Nowy
Komentarze (0)
Na razie nie dodano żadnej recenzji.

9 innych produktów w tej samej kategorii:

RNase R
Add to Compare

RNase R

RNase R is an E. coli exoribonuclease which exhibits 3' to 5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3 overhangs (less than seven nucleotides).

RNAse Inhibitor
Add to Compare
MC Lab

RNAse Inhibitor

Inhibitor RNazowy to kwasowe białko o masie 52 kDa, które jest silnym inhibitorem niekonkurencyjnym rybonukleaz typu trzustkowego, takich jak RNaza A, RNaza B i RNaza C.