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Description:
RNase I catalyzes the hydrolysis of single-stranded RNA to nucleoside 3'-monophosphates via 2', 3' cyclic monophosphate intermediates. Note: The enzyme is inactivated by heating at 70°C for 15 minutes, eliminating phenol extractions to remove the enzyme.
Application:
- Degradation of single-stranded RNA to mono-, di- and trinucleotide
- Used in ribonuclease protection assays
Source:
An E. coli strain containing a genetic fusion of the RNase I gene (rna) from E. coli and the gene coding for maltose-binding protein (MBP).
Unit Definition:
One unit of enzyme required to catalyze the degradation of 100 ng of E. coli ribosomal RNA per second into acid-soluble nucleotides at 37°C.
Storage Buffer:
Supplied in 50% Glycerol, 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.01 mM EDTA.
Recommended Storage Condition: -20°C
RNIE-100
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