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Description
RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules. RNase H digestion produces ribonucleotide molecules with 5'-phosphate and 3'-hydroxyl termini. RNAse H is nearly inactive against single or double-stranded RNA molecules.
RNase H from the thermophilic bacterium Bacillus stearothermophilus is thermostable, has optimal activity above 65°C and making it useful for a broad range of applications.
Applications
Removal of poly(A) tails of mRNA hybridized to poly(dT)
Removal of mRNA during second strand cDNA synthesis
Source
A recombinant E. coli strain carrying the RNAse H gene from the thermophilic bacterium Bacillus stearothermophilus
Supplied in
20 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.1 mM EDTA, 0.1 mM DTT, 50% Glycerol, pH 7.9 @ 25°C
Supplied with
10x RNAse H Buffer
10x RNAse H Buffer
500 mM Tris-HCl, 750 mM KCl, 30 mM MgCl2, 100 mM DTT, pH 8.3 @ 25°C
Unit Definition:
1 unit is defined as the amount of enzyme that will hydrolyze 1 nmol of RNA from an 3H-labeled DNA:RNA hybrid molecule into acid-soluble material in 20 minutes at 37°C.
Recommended Storage Condition: -20°C
RNHB-100
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RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules.
Ostatnio przeglądane
RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules.