Golden Gate Ligase

Description:
Golden Gate Ligase is a recombinant enzyme designed for efficient joining of DNA fragments with either sticky or blunt ends. The manufacturer describes it as a premium-grade enzyme. It contains an increased amount of active enzyme and is supplied with 10x Start solution, which, according to the manufacturer, enables effective ligation even at low substrate concentrations or with DNA ends that are difficult to join. The enzyme is designed, among other uses, for cloning with the Golden Gate Assembly technique, which allows multiple DNA fragments to be cloned simultaneously. According to the manufacturer, this shortens the workflow and increases process efficiency. The manufacturer also states that it offers higher ligation efficiency even under challenging conditions, broad applicability across most cloning protocols, and stable, reproducible performance.

Features:

• Recombinant enzyme supplied as a solution
• Concentration 5 U/µl
• Joins DNA fragments with sticky or blunt ends
• Increased amount of active enzyme
• Supplied with 10x Start solution
• Storage buffer: 20 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol (v/v)
• Storage at -20 °C
• For research use only

Applications:

• Golden Gate Assembly: cloning of multiple DNA fragments in a single reaction using a type IIS restriction enzyme
• Conventional cloning with restriction enzymes: ligation of DNA fragments with sticky or blunt ends
• Adapter ligation for sequencing library preparation

Kit contents:

• Golden Gate Ligase (-20 °C)
• 10x Start solution (-20 °C)