Supreme TC Grade Agar

Description:
Supreme TC Grade Agar is a high-quality gelling agent ideal for use in plant tissue cultures. It provides a solid base for the medium and ensures uniform distribution of nutrients and hormones, supporting optimal plant growth and development during in vitro cultivation. It is a popular choice due to its ease of use, high purity, and consistent results.

Features:

• Easy to use in the laboratory
• Provides stable support for the growth and development of explants
• Enables efficient nutrient absorption by plant cells
• Cost-effective and efficient

Instructions for use:
To prepare 1 liter of medium for tissue cultures, follow these steps:

1. Add 4.54 g of PCT’s MS Media (or other suitable medium), 30 g of sugar, and PPM™ (typically 1–2 ml per liter) to 800 ml of distilled water.
2. Top up with water to a final volume of 1,000 ml.
3. Adjust the pH of the medium to between 5.4–5.8.
4. Add 6–8 g of Supreme TC Grade Agar and mix well.
5. Sterilize in an autoclave for 20 minutes at 15 psi and 121°C.
6. Your medium is now ready to use.

Applications:

• Thickening agent and carrier for microbial and plant culture media

• Essential component for in vitro rooting, growth, and differentiation of explants

• Dosage: 0.5–1.0% (w/v) for solidifying the medium

Video tutorial:

How to Make Tissue Culture Agar - Gelling Agent

Available sizes:

100 g (cat.no. A100)
500 g (cat.no. A500)
1 kg (cat.no. A1000)
5 kg (cat.no. A5000)
25 kg (cat.no. A2500)