DKW Medium w/ Vitamins
Plant Cell Technology

DKW Medium w/ Vitamins

€61.40
Tax excluded

Universal application
Enhanced growth
Simple preparation

Volume: 10 l
€61.40
Tax excluded
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Description:

Originally developed for the micropropagation of walnut (Juglans spp.) from nodal explants, DKW Medium is now widely used for the in vitro cultivation of woody species, herbs, and recalcitrant plants. Its distinct formulation features lower nitrogen content and higher sulfate levels, supporting plant vitality and development under tissue culture conditions.

 

Features:

  • Complete mineral and vitamin composition for robust explant growth
  • Easy to prepare and versatile for multiple plant types
  • Supports shoot proliferation and regeneration from callus
  • Optimized for woody plants and challenging species (e.g., turmeric, walnut)

 

Recommended use:

  • Micropropagation of woody and herbaceous plants
  • Shoot induction and regeneration from callus
  • Suitable for recalcitrant species requiring tailored media composition
 

Preparation Instructions:

  1. Dissolve 5.36 g of DKW powder in 800 ml of distilled water
  2. Add 30 g sucrose and PPM™ (1–2 ml/l)
  3. Adjust volume to 1 liter with distilled water
  4. Adjust pH to 5.4–5.8

  5. Add a gelling agent:

    Agar (6–8 g/l) or
  6. Gellan gum (3–4 g/l)
  7. Sterilize in an autoclave for 20 minutes at 121 °C, 15 psi
  8. Medium is ready for use
 

Applications:

  • In vitro propagation of woody plants, herbs, and difficult-to-root species
  • Shoot multiplication and callus regeneration
  • Enhancing success rates for specialized plant tissue culture protocols

 

Video tutorial:

 

Available sizes:

1 L (Cat. No. DKW1L)
10 L (Cat. No. DKW10L)
50 L (Cat. No. DKW50L)
100 L (Cat. No. DKW100L)

DKW10L
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