Taq-Klenow Polymerase

Description:
Taq-Klenow Polymerase is a modified form of Taq DNA polymerase generated by N-terminal truncation. Compared to full-length Taq, it lacks 5’→3’ exonuclease activity and is optimized for DNA amplification and enzymatic reactions requiring stable polymerase activity. Its structure provides improved stability and modified enzymatic properties suitable for PCR and related applications.

Features:

• truncated Taq DNA polymerase
• 5’→3’ DNA polymerase activity
• lacks 5’→3’ exonuclease activity
• suitable for PCR and enzymatic assays
• improved enzyme stability
• recombinant enzyme

Source:

• recombinant enzyme expressed in Escherichia coli
• derived from Thermus aquaticus

Unit Definition:
One unit (U) is defined as the amount of enzyme that incorporates 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 75 °C.

Applications:

• PCR amplification
• primer extension
• microarray analysis
• DNA detection and analytical workflows
• molecular biology research