10X Thermal Chelator

Description:
10X Thermal Chelator is a patented reagent designed to create a thermally reversible Hot-Start/Cold-Stop PCR system without chemical or antibody modification of DNA polymerases. Its mechanism is based on reversible chelation of magnesium ions (Mg²⁺), an essential cofactor for DNA polymerases. At low temperatures, Mg²⁺ ions are bound, keeping the polymerase inactive. Upon heating, magnesium ions are released, allowing PCR amplification to proceed. Cooling the reaction causes Mg²⁺ to be chelated again, stopping the reaction. No enzyme activation step is required.

Features:

• 10× concentrated reagent
• patented reversible Mg²⁺ chelation technology
• Hot-Start / Cold-Stop PCR system
• no chemical modification of DNA polymerase required
• no heat activation step required
• compatible with thermostable DNA polymerases
• reduces non-specific amplification and primer-dimer formation

Applications:

• Hot-Start PCR
• standard PCR
• improved PCR specificity
• reduced non-specific amplification
• molecular biology applications