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Q Cross-linked Agarose High Performance
Q Cross-linked Agarose High Performance has well-deserved reputations as highly successful anion and cation ion exchange media for purifying a wide range of biomolecules.
Description:
Glutathione S-transferase (GST) gene fusion systems have been widely used for obtaining large amounts of desirable protein in Escherichia coli. The fusion protein, which contains a GST tail can then be purified through affinity chromatography. MCLAB’s glutathione agarose is designed for the specific purification of GST recombinant proteins and other glutathione-binding proteins. Glutathione agarose is uniquely formulated for excellent binding capacity and purity of the protein of interest.
Specifications
|
Chromatography technique |
GST-tagged protein purification |
|
Matrix |
Highly cross-linked 6% beaded agarose |
|
Active group |
Glutathione |
|
Active group density |
>40 umol/ml drained medium |
|
Spacer |
1,4-bis(2,3-epoxypropoxy)butane (12 atom stable and uncharged ether hydrophilic linkage) |
|
Bead geometry & size |
Spherical 50 to 150 um |
|
Bead mean diameter d50v |
90 um |
|
Linear flow velocity |
<75 cm/h at 25 °C, HR 16/10 column, 5 cm bed height |
|
Recommended linear flow rate |
<30 cm/h |
|
Pressure/flow specification |
Base matrix 100-200 cm/h, pressure drop cm H2O/bed height = 15, bed height 10 cm, 5 cm i.d. |
|
1ml Cartridge bed dimensions |
7×25 mm |
|
1ml Cartridge bed height |
25 mm |
|
1ml Cartridge bed volume |
1 ml |
|
1ml Cartridge column I.D |
7 mm |
|
5ml Cartridge bed dimensions |
16×25 mm |
|
5ml Cartridge bed height |
25 mm |
|
5ml Cartridge bed volume |
5 ml |
|
5ml Cartridge column I.D |
16 mm |
|
Maximum cartridge flow rate |
5 ml/min (1 ml cartridge) or 20 ml/min (5ml cartridge) |
|
Recommended cartridge flow rate |
1 ml/min (1 ml cartridge) or 5 ml/min (5 ml cartridge) |
|
Maximum pressure during operation |
5 bar [0.5 MPa] (70 psi) |
|
pH Stability working range |
3 to 13 |
|
pH Stability cleaning in place (cip) |
2 to 14 |
|
Chemical stability |
Stable to commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride, Stable in organic solvents, e.g. 50% methylformamide and 50% dioxane. |
|
Storage |
2 to 8 °C |
GAB-CC1x5
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