Description
SP Cross-linked Agarose Big Beads are strong ion exchangers designed for industrial applications. The large particle sizes (100-300 µm) and excellent physical stability of the base matrix ensures maintained speed, even with viscous samples. SP Cross-linked Agarose Big Beads are therefore the ultimate ion exchange media for initial purifications when high viscosity precludes the use of ion exchangers with smaller bead size, such as Cross-linked Agarose Fast Flow ion exchangers. The unique flow characteristics are also invaluable when adsorption needs to be done quickly.
Features
Easy to scale-up
High flow rates
High chemical resistance for effective cleaning-in-place (CIP)
Easy maintenance
Specification
| Ion exchanger type | Sulfopropyl strong cation |
| Ionic capacity | 0.18-0.25 mmol (H+)/ml |
| Dynamic capacity | 70 mg RNase/ml medium |
| Pressure/flow spec. | 1200-1800 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm |
| Average particle size | 200 μm (100-300 μm) |
| Matrix | Highly cross-linked agarose, 6% |
| pH stability | 3-14 (short term), 4-12 (long term) |
| Chemical stability | Stable in all common aqueous buffers: 8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH*, and 1 M acetic acid*. |
| Storage | 20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP) |
| Storage temperature | 4°C to 30°C |
Used in proteomics and protein chromatography, amino acid agarose is prepared by covalently coupling of amino acid through a 17 atom stable and uncharged ether hydrophilic linkage to highly cross-linked 6% agarose with bead mean diameter of 90 um.
Epoxy-activated Agarose is a pre-activated affinity chromatography support for the immobilization of a variety of biomolecules that contain nucleophiles, such as hydroxyl, amino, or thiol groups. These groups couple to the epoxy groups on the support, which is then used for the purification of proteins, carbohydrates, or DNA.
Glutathione S-transferase (GST) gene fusion systems have been widely used for obtaining large amounts of desirable protein in Escherichia coli.
Q Cross-linked Agarose High Performance has well-deserved reputations as highly successful anion and cation ion exchange media for purifying a wide range of biomolecules.
Recombinant Protein A is an immunoglobulin-binding protein derived from the cell wall of the bacterium Staphylococcus aureus.
Ni2+, Co2+, Cu2+, or Zn2+ charged nitrilotriacetic acid (NTA) coupled to Agarose CL-6B via a stable and uncharged long ether hydrophilic spacer arm, and offers high binding capacity and minimal non-specific binding.
Glutathione S-transferase (GST) gene fusion systems have been widely used for obtaining large amounts of desirable protein in Escherichia coli.
SP Cross-linked Agarose Big Beads are strong ion exchangers designed for industrial applications. The large particle sizes (100-300 µm) and excellent physical stability of the base matrix ensure maintained speed even with viscous samples.
Čeština
Polski
