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Glutathione Agarose Cartridge
Glutathione S-transferase (GST) gene fusion systems have been widely used for obtaining large amounts of desirable protein in Escherichia coli.
Description
SP Cross-linked Agarose Big Beads are strong ion exchangers designed for industrial applications. The large particle sizes (100-300 µm) and excellent physical stability of the base matrix ensures maintained speed, even with viscous samples. SP Cross-linked Agarose Big Beads are therefore the ultimate ion exchange media for initial purifications when high viscosity precludes the use of ion exchangers with smaller bead size, such as Cross-linked Agarose Fast Flow ion exchangers. The unique flow characteristics are also invaluable when adsorption needs to be done quickly.
Features
Easy to scale-up
High flow rates
High chemical resistance for effective cleaning-in-place (CIP)
Easy maintenance
Specification
| Ion exchanger type | Sulfopropyl strong cation |
| Ionic capacity | 0.18-0.25 mmol (H+)/ml |
| Dynamic capacity | 70 mg RNase/ml medium |
| Pressure/flow spec. | 1200-1800 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm |
| Average particle size | 200 μm (100-300 μm) |
| Matrix | Highly cross-linked agarose, 6% |
| pH stability | 3-14 (short term), 4-12 (long term) |
| Chemical stability | Stable in all common aqueous buffers: 8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH*, and 1 M acetic acid*. |
| Storage | 20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP) |
| Storage temperature | 4°C to 30°C |
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