Description
RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules. RNase H digestion produces ribonucleotide molecules with 5'-phosphate and 3'-hydroxyl termini. RNAse H is nearly inactive against single or double-stranded RNA molecules.
RNase H from the thermophilic bacterium Bacillus stearothermophilus is thermostable, has optimal activity above 65°C and making it useful for a broad range of applications.
Applications
Removal of poly(A) tails of mRNA hybridized to poly(dT)
Removal of mRNA during second strand cDNA synthesis
Source
A recombinant E. coli strain carrying the RNAse H gene from the thermophilic bacterium Bacillus stearothermophilus
Supplied in
20 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.1 mM EDTA, 0.1 mM DTT, 50% Glycerol, pH 7.9 @ 25°C
Supplied with
10x RNAse H Buffer
10x RNAse H Buffer
500 mM Tris-HCl, 750 mM KCl, 30 mM MgCl2, 100 mM DTT, pH 8.3 @ 25°C
Unit Definition:
1 unit is defined as the amount of enzyme that will hydrolyze 1 nmol of RNA from an 3H-labeled DNA:RNA hybrid molecule into acid-soluble material in 20 minutes at 37°C.
Recommended Storage Condition: -20°C
RNase R is an E. coli exoribonuclease which exhibits 3' to 5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3 overhangs (less than seven nucleotides).
RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules.
The Blunting Express Kit can be used to generate blunt-ended DNA fragments for subsequent use in ligation, cloning, cDNA construction, and MCLAB’s Topomize Amplicon Library Prep Kit (Cat# TopoA-50A or TopoA-50B). Our proprietary enzyme mix effectively fills in 5’ overhangs and eliminates A-tailed 3’ overhangs on the template.
RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules.
Čeština
Polski
