RNase R, E. coli

Description
RNase R is an E. coli exoribonuclease which exhibits 3' to 5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3 overhangs (less than seven nucleotides). RNase R is ideally suited to the study of lariat RNA produced by traditional splicing, as well as circRNAs which arise through back-splicing. By removing linear RNAs from cellular or RNA extracts, RNase R greatly facilitates the identification of circular species through RNA-sequencing.

Applications

Alternative splicing studies

Gene expression studies

Intron cDNA production

Intronic screening of cDNA libraries

Isolation of splicing intermediates and lariats

Source:
A recombinant E. coli strain carrying the RNAse R gene from E. coli

Storage Buffer:
50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 0.1% Triton® X-100, 1 mM DTT, 50% glycerol

10X RNase R Reaction Buffer:
0.2 M Tris-HCl (pH 8.0), 1 M KCl, 1 mM MgCl₂

Unit Definition: One unit converts 1 μg of poly-r(A) into acid-soluble nucleotides in 10 minutes at 37°C in 20 mM Tris-HCl (pH 8.0), 100 mM KCl and 0.1 mM MgCl₂.

Storage Condition:  -20°C