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Tn5 Transposase
origin: E. coli carrying the Tn5 gene
random transposon insertion into DNA
requires 19-bp ME sequence
Koszyk
Description:
The enzyme mix was developed to generate random fragmentation of genomic DNA with blunt ends in a single step. The fragmented DNA can be used for applications such as Next-Generation Sequencing (NGS), blunt end ligation and PCR cloning.
Compared to mechanical shearing methods, enzymatic DNA fragmentation is significantly easier and more efficient. It allows convenient handling of multiple samples simultaneously, minimizes sample loss, and eliminates the need for expensive equipment.
The illustration below shows the workflow for DNA fragmentation for NGS:
Step 1: DNA fragmentation – 1 minute hands-on, 30 minutes incubation
Step 2: Magnetic bead purification – 2 minutes hands-on, 6 minutes automated incubation
This streamlined process saves time, allowing most of the workflow to run unattended.
The graph demonstrates how DNA fragmentation progresses depending on incubation time using this enzymatic mix. Each curve represents a different incubation duration (10, 15, 20, 25 minutes) and the resulting average DNA fragment sizes (204–313 bp). The product allows precise control over fragment size – longer incubation yields smaller DNA fragments.
Features:
- Fast fragmentation: Efficient enzymatic DNA fragmentation in 30–45 minutes
- Ease of use: Only 3 minutes hands-on time – minimal manual steps
- Sample flexibility: Compatible with EDTA-free DNA and DNA in TE buffer
- No expensive equipment needed: No need for complex mechanical shearing systems
- NGS-optimized: Ideal for NGS library prep, PCR cloning, TA-ligation, and blunt-end ligation without detectable sequencing bias
- Fragment size control: Easily adjusted by changing incubation time – longer incubation results in shorter fragments
Product sizes:
50 reactions (Cat. No. 40063S)
200 reactions (Cat. No. 40063L)
40063S
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DNA Fragmentation & A-tailing Enzyme Mix
Fast and simple fragmentation
Fragment size easily controlled by incubation time
Ideal for NGS library preparation
Fast and simple fragmentation
Fragment size easily controlled by incubation time
Ideal for NGS library preparation