Description:
Thermostable RNase H has an optimal activity above 65°C and can be used up to 95°C. The enzyme degrades RNA in a DNA:RNA hybrid, maximizing sensitivity and selectivity without affecting DNA or unhybridized RNA.
Application:
- High hybridization stringency
- Specific hydrolysis of RNA in a DNA:RNA hybrid
- Diagnostic assay of DNA sequences by isothermal probe amplification
- Mapping of mRNA structures
Source:
A recombinant protein purified from E. coli, cloned the gene encoding the Thermus thermophilus RNase H.
Unit Definition:
One unit of the enzyme results in the acid-solubilization of 1 nmol of polyadenylic acid in the presence of an equimolar concentration of polythymidylic acid in 20 minutes at 45°C in 50 mM Tris-HCl(pH 7.5), 100 mM NaCl, and 10 mM MgCl2. Note: The unit assay is performed at 45°C because this is optimal for the Tm of poly(dT):poly(A). The optimal temperature for many applications may be considerably higher.
10X RNase H Reaction Buffer
500 mM Tris-HCl(pH 7.5)
1 M NaCl
100 mM MgCl2
pH 7.6 @ 25°C
Storage Buffer:
Supplied in 50% Glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1.0 mM DTT, 0.1 mM EDTA, and 0.1% Triton X-100.
Recommended Storage Condition: -20°C
Inhibitor RNazowy to kwasowe białko o masie 52 kDa, które jest silnym inhibitorem niekonkurencyjnym rybonukleaz typu trzustkowego, takich jak RNaza A, RNaza B i RNaza C.
RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules.
RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules.
RNase R is an E. coli exoribonuclease which exhibits 3' to 5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3 overhangs (less than seven nucleotides).
MCNext DNA Sample Prep Kit jest wysokiej jakości, korzystnym cenowo zestawem do przygotowania próbki do NGS za pomocą platformy Illumina.
RNase R is an E. coli exoribonuclease which exhibits 3' to 5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3 overhangs (less than seven nucleotides).
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