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- Nucleic Acid Analysis
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Enzymes
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- Mesophilic DNA Polymerases
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- Chaperon Proteins and Disulfide Bond Isomerase
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News
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1st Czechoslovak Congress of Medical Genetics 2025
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
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RANK 2025
Visit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...
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Kapras Day 2025
We would like to invite you to the 23rd Kapras Day on the topic of "Clinical Genetics," which will take place on Wednesday, February 26, 2025, in the Congress Hall of Hotel Olšanka in Prague. We loo...
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Description
Epoxy-activated Agarose is a pre-activated affinity chromatography support for the immobilization of a variety of biomolecules that contain nucleophiles, such as hydroxyl, amino, or thiol groups. These groups couple to the epoxy groups on the support, which is then used for the purification of proteins, carbohydrates, or DNA.
Epoxy-activated Agarose CL-6B is based on highly cross-linked agarose 6% beads which have structural characteristics that result in superb physical strength, excellent flow properties, low backpressure, and an open pore structure. The open pore structure allows coupling of large ligands for the purification of large targets, including protein A for purification of monoclonal antibodies, and other ligands for purification of recombinant proteins, such as those containing maltose-binding protein and calmodulin. In addition, Epoxy-activated Agarose CL-6B allows operation at very high flow rates, resulting in increased throughput and rapid column cleaning between chromatography runs. It is supplied as a semi-dry powder (1 g of powder gives ~3.3 ml final drained medium) to significantly shorten conditioning time before ligand coupling.
Specifications
|
Chromatography technique |
Affinity coupling |
|
Application |
Resin with a long hydrophilic spacer arm particular suitable for immobilization of small |
|
Matrix |
molecules as well as proteins |
|
Active group |
Highly cross-linked 6% beaded agarose |
|
Active group density |
>40 umol/ml drained medium |
|
Spacer |
1,4-bis(2,3-epoxypropoxy)butane (12 atom stable and uncharged ether hydrophilic linkage) |
|
Group to be coupled |
-OH, -NH2, or -SH |
|
Bead geometry & size |
Spherical 50 to 150 um |
|
Bead mean diameter d50v |
90 um |
|
Linear flow velocity |
<75 cm/h at 25 °C, HR 16/10 column, 5 cm bed height |
|
Recommended linear flow rate |
<30 cm/h |
|
Pressure/flow specification |
Base matrix 100-200 cm/h, pressure drop cm H2O/bed height = 15, bed height 10 cm, 5 cm i.d. |
|
pH Stability working range |
3 to 13 |
|
pH Stability cleaning in place (cip) |
2 to 14 |
|
Chemical stability |
Stable to commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride, Stable in organic solvents, e.g. 50% methylformamide and 50% dioxane. |
|
Storage |
2 to 8 °C |
|
Name |
Cat# |
Description |
|
Epoxy-activated Agarose |
EPA-50 |
15g epoxy-activated agarose CL-6B (semi-dry resin swelling to 50 ml drained medium) |
|
Epoxy-activated Agarose |
EPA-250 |
75g epoxy-activated agarose CL-6B (semi-dry resin swelling to 250 ml drained medium) |
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