Formamide, deionized, sequencing grade, 25mls

TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help to protect DNA and RNA.

LB Agar plates contain 1.0% Tryptone, 0.5% yeast extract, 0.5% NaCl, 1.5% agar.

Sodium hydroxide is commonly used in the clandestine production of methamphetamine to change the pH of the solution to a basic pH so that it can become soluble in an organic solution.

5M solution of sodium chloride (NaCl) in high purity dH₂O. Solution is 0.2 μm filtered and dispensed into durable, square Nalgene bottles.

Bovine Serum Albumin (BSA) has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions. MCLAB’s Ultrapure BSA is a "non-acetylated" BSA, pure enough to use when the integrity of DNA or RNA is essential. It has been tested for DNase, RNase, endonuclease, protease, peroxidase, and alkaline phosphatase activity, and assayed for fluorescence background.

Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation.

Buffer EB is the elution buffer used in many Qiagen kits, e.g. PCR, Gel Extraction, Nucleotide Removal Kits, MinElute Kits  and the QIAprep Miniprep Kits for DNA cleanup or small-scale plasmid purification. The purified DNA can also be eluted in TE (10 mM Tris-Cl, 1 mM EDTA, pH 8.0), but the EDTA may inhibit certain subsequent enzymatic reactions.

Acetylated BSA is used as a carrier protein and an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is vital. The acetylation process inactivates any trace of active nuclease activity. While the acetylation process does change BSA's binding characteristics and makes it inhibitory in PCR reactions, it is normally added to restriction digests.

DEPC-treated Water is deionized, high quality, molecular biology grade water.

Used to select for ampicillin resistance in mutated and transformed cells.

CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate ) is a zwitterionic detergent used to solubilize proteins. It is used as a non-denaturing solvent in the process of protein purification and is especially useful in purifying membrane proteins.

HEPES Solution is a biological buffer useful in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture, and degree of chemical definition required for the cell culture application.

Kanamycin Sulfate solution is an aminoglycoside antibiotic consisting of a six-membered aminocyclitol ring attached to aminosugar side chains through glycosidic bonds. Kanamycin Sulfate solution acts by inhibiting protein synthesis, and compromising translational fidelity in susceptible bacteria.

MCLAB’s 7M Guanidine-HCl Solution is a ready-to-use solution of guanidine hydrochloride, which can be easily diluted and pH-adjusted to any concentration below 7M.

• DNA cleanup
• high efficiency