Bst DNA Polymerase (regular)

Description:
rBst DNA Polymerase is the product of the DNA poll gene of the thermophilic bacterium Bacillus stearothermophilus (Bst). As an enzyme, it contains the 5´ -> 3´ polymerase activity, but lacks the 5´ -> 3´ exonuclease domain. It also has optimal activity at 65°C and can be used to synthesize DNA in regions that containing template secondary structure or high GCs where other non-thermostable DNA polymerases may fail in sequencing. Therefore, rBst DNA Polymerase is useful in replicating difficult templates in various applications.

Application:
- Isothermal DNA amplification by the method of:
· loop-mediated isothermal amplification (LAMP)
· whole genome amplification (WGA)
· ramification amplification (RAM)
- Random-primed DNA labeling
- Labeling by fill-in 5'-overhangs of dsDNA

Source:
An E. coli strain that contains the gene from Bacillus stearothermophilus.

Supplied in:
10 mM Tris-HCl
50 mM KCl
1.0 mM DTT
0.1 mM EDTA
0.1% Triton X-100
50% Glycerol
pH 7.5 @ 25°C

Supplied with:
10X PCR buffer II

10x PCR Buffer ll:
200 mM Tris-HCl
100 mM Ammonium Sulfate
100 mM KCl
20 mM MgSO₄
1.0% Triton X-100
pH 8.8 @ 25°C

Unit Definition:
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C.

Recommended Storage Condition: -20°C

Reference:
Kiefer, et al. Structure 15 January 1997. 5, 95-108.