T4 DNA Polymerase

Description:
T4 DNA Polymerase catalyzes the extension of a primed DNA template in the 5' -> 3' direction. This enzyme exhibits a powerful 3' -> 5' exonuclease activity, while lacking any inherent 5' -> 3' exonuclease or strand displacement functions.

Specific Activity: 5,555U/mg

Application:
- 3'-overhang removal to form blunt ends
- 5'-overhang fill-in to form blunt ends
- Single strand deletion for sub-cloning
- Second strand synthesis in site-directed mutagenesis
- Probe labeling using replacement synthesis

Source:
Purified from a strain of E. coli that expresses the recombinant T4 DNA Polymerase gene.

Supplied in:
100 mM KPO₄
1.0 mM DTT
0.1 mM EDTA
50% Glycerol
pH 6.5 @ 25°C

Supplied With: 10x Blue Buffer

10x Blue Buffer:
500 mM NaCl
100 mM Tris-HCl
100 mM MgCl₂
10 mM DTT
pH 7.9 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-precipitable material in 30 minutes at 37°C.

Recommended Storage Condition: -20ºC