Phi29 DNA Polymerase

Phi29 DNA Polymerase is a replicative polymerase. This enzyme has  assist DNA replication, phi29 DNA Polymerase has the highest processivity and strand displacement activity among known DNA polymerases - more than 70 kb long DNA stretches can be synthesized.

Description:
Phi29 DNA Polymerase is responsible for the replication of the Bacillus Subtilis phage phi29. The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand displacement activity and a 3' -> 5' proofreading exonuclease function.

Specific Activity: 1,000,000U/mg

Application:
- Catalyzes the removal of 5'-mononucleotides from duplex DNA
- Replication requiring a high degree of strand displacement and/or processive synthesis
- High fidelity replication at moderate temperatures

Source:
A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.

Supplied in:
10 mM Tris-HCl
100 mM KCl
0.1 mM EDTA
1 mM DTT
0.5% Tween-20
0.5% NP-40
50% Glycerol
pH 7.4 @ 25°C

Supplied with:
10x phi29 DNA Polymerase Reaction Buffer

10x phi29 DNA Polymerase Buffer
500 mM Tris-HCl
100 mM (NH₄)₂SO₄
40 mM DTT
100 mM MgCl₂
pH 7.5 @ 25°C

Unit Definition:
1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dTTP into acid insoluble material in 10 minutes at 30°C.

Recommended Storage Condition: -20°C