5´ DNA Adenylation Kit

5´ DNA Adenylation Kit

5´ DNA Adenylation Kit is for the enzymatic synthesis of 5’ adenylated ssDNA linkers.

Description
5´ DNA Adenylation Kit is for the enzymatic synthesis of 5’ adenylated ssDNA linkers. The kit is optimized to produce the adenylated DNA with or without 3´ terminator. The 5´ DNA adenylation kit can simply and efficiently generate greater than 95% conversion of pDNA to AppDNA.

 

Features

  • One step reaction. Simple and efficient.
  • 65°C reaction temperature reduces secondary structural concerns.
  • Highly efficient process eliminates the need for purification of the product.
  • Easily scale up from pmol to µmol range.

Application
Enzymatic 5´adenylation of single-stranded DNA linkers for next generation sequencing (NGS).


5 DNA Adenylation Kit


Kit Components
10X 5’ DNA Adenylation Reaction Buffer
           500 mM Sodium Acetate (pH 6.0 @ 25°C)
           100 mM MgCl2 
           50 mM DTT 
           1 mM EDTA
1 mM ATP
Mth RNA Ligase

 

Storage Temperature:-20°C

APPK-100
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