The 2X MCAmp library amplification master mix has been specially formulated to provide accurate and robust amplification of NGS (next-generation sequencing) library fragments.
Description
The 2X MCAmp library amplification master mix has been specially formulated to provide accurate and robust amplification of NGS (next-generation sequencing) library fragments. The master mix utilizes a proprietary combination of our ultra-fast, high-fidelity DNA polymerases. The polymerases all possess 3'->5' exonuclease (proofreading) activity with a processivity ten times greater than generic DNA polymerases. The reaction buffer has been optimized to provide robust amplification of library fragments even with low input and GC or AT rich sequences. The resulting amplifications have robust yield with high-fidelity, low PCR duplication, and low bias which produces greater and more uniform coverage especially in challenging genomic regions.
For Illumina® platforms, an optimized 10X Primer Set targeting the P5 and P7 sites is also available.
Product highlights
High-fidelity: The master mix utilizes DNA polymerases that all possess 3'->5' exonuclease (proof-reading) activity for accurate nucleotide incorporations into your final products.
High-efficiency: The polymerases in the master mix have ultra-high processivity so that less cycles are required for sequence ready libraries.
Low bias and PCR duplication: Optimized buffer formulation to minimize amplification bias and PCR duplication. This results in a greater number of reads with more uniform coverage, helping to minimize cost and time.
Recommended storage condition: -20°C
Heat Inactivation: No
Unit Assay Conditions:
25 mM TAPS-HCl (pH 9.3 @ 25°C), 50 mM KCl, 2 mM MgCl2, 1 mM β-mercaptoethanol, 200 μM dNTPs including [3H]-dTTP and 400 μg/ml activated Calf Thymus DNA.
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C.
Figure : Library preparation and amplification results from varying input quantities of sheared human genomic DNA. Library preparation and size selection (~350 bp) was carried out using MCLAB's Topomize DNA Library Prep Kit. All amplifications were carried out to eight cycles. A. Agarose gel image after library amplification and clean up showing stable size distribution. M: 100 bp marker. Lane 1: 100 ng input DNA. Lane 2: 50 ng input DNA. Lane 3: 25 ng input DNA. B. Average DNA yield of corresponding gel agarose samples determine by qPCR using MCNexttm SYBR® Fast qPCR Library Quantifiaction Kit.
5´ DNA Adenylation Kit is for the enzymatic synthesis of 5’ adenylated ssDNA linkers.
MCMax Cell-Free DNA Extraction Kit is designed to purify cell-free DNA (cfDNA) from 0.2–10 ml plasma samples. The kit contains everything necessary for purification, with no preprocessing required. The purified cell-free DNA is of high quality with little genomic DNA contamination and is ready for use in downstream applications such as qPCR, next-generation sequencing and digital PCR.
MCMag™ Library Purification kit is a highly efficient purification system with size selection for NGS library after PCR enrichment.
The Blunting Express Kit can be used to generate blunt-ended DNA fragments for subsequent use in ligation, cloning, cDNA construction, and MCLAB’s Topomize Amplicon Library Prep Kit (Cat# TopoA-50A or TopoA-50B). Our proprietary enzyme mix effectively fills in 5’ overhangs and eliminates A-tailed 3’ overhangs on the template.
The 2X MCAmp library amplification master mix has been specially formulated to provide accurate and robust amplification of NGS (next-generation sequencing) library fragments.
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