Description:
The MCLAB's Fragmented DNA End Repair Kit is used for repairing fragmented DNA ends by sonication, nebulization or nucleases. The kit has been optimized to the maximum efficiency and convenience in DNA sample preparation workflow for next-generation sequencing (including Illumina®Genomic DNA Sample Prep protocol, Roche 454TM Library Preparation and Life Technologies SOLiDTM Library Preparation).
Function:
The superior function of the Fragmented DNA End Repair Kit depends on MCLAB's proprietary enzyme systems. Unique improvement to each key enzyme increases sensitivity, flexibility and speed to next-generation sequencing. The DNA fragment is converted to the blunt-ended DNA with a 5'-phosphate. DNA repaired by MCLAB's Fragmented DNA End Repair Kit can be used directly for blunt end cloning or blunt-ended adaptor ligation, or be converted to DNA with 3´ dA-tail (MCLAB dA-Tailing kit, Cat #: NGDT-100) for subsequent ligation (MCLAB DNA Ligation kit, Cat #: NGDL-100).
Features:
- Simplified workflow, reduced hands-on time
- Optimized reaction conditions, increased efficiency
- Up to 10µg fragmented DNA
- Automation friendly format
Components:
Sufficient reagents are supplied in the Fragmented DNA End Repair Kit to convert 20 or 100 fragmented DNA samples to 5´-phosphorylated, blunt ended DNA.
Fragmented DNA End Repair Kit (20 reactions):
End Repair Enzyme Mix: 120µl
End Repair Buffer: 400µl
Fragmented DNA End Repair Kit (100 reactions):
End Repair Enzyme Mix: 600µl
End Repair Buffer: 2ml
Recommended Storage Condition: -20oC
The Blunting Express Kit can be used to generate blunt-ended DNA fragments for subsequent use in ligation, cloning, cDNA construction, and MCLAB’s Topomize Amplicon Library Prep Kit (Cat# TopoA-50A or TopoA-50B). Our proprietary enzyme mix effectively fills in 5’ overhangs and eliminates A-tailed 3’ overhangs on the template.
5´ DNA Adenylation Kit is for the enzymatic synthesis of 5’ adenylated ssDNA linkers.
MCMax Cell-Free DNA Extraction Kit is designed to purify cell-free DNA (cfDNA) from 0.2–10 ml plasma samples. The kit contains everything necessary for purification, with no preprocessing required. The purified cell-free DNA is of high quality with little genomic DNA contamination and is ready for use in downstream applications such as qPCR, next-generation sequencing and digital PCR.
The 2X MCAmp library amplification master mix has been specially formulated to provide accurate and robust amplification of NGS (next-generation sequencing) library fragments.
MCMag™ Library Purification kit is a highly efficient purification system with size selection for NGS library after PCR enrichment.
36 discrete DNA fragments (20 to 600 bp)
High compatibility
High accuracy
Čeština
Polski
