LE-100 5,000 units, 20,000 U/ml 120 €
LE-200 20,000 units 20,000 U/ml 361 €
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Lambda Exonuclease catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate.
Description:
Lambda Exonuclease is a highly processive exonuclease which selectively degrades the 5'-phosphorylated strand of double-stranded DNA via the stepwise 5' to 3' release of mononucleotides from duplex DNA. Lambda Exonuclease is inactive against 5'-hydroxyl termini(1), and will not initiate excision at a nick or gap(2), though it will degrade a 5'-overhanging tail from duplex DNA at a greatly reduced rate.
Specific Activity: 100,000 U/mg
Application:
Catalyzes the removal of 5'-mononucleotides from duplex DNA.
Source:
Purified from a strain of E. coli that overexpresses the exonuclease gene from bacteriophage Lambda.
Supplied in:
25 mM Tris-HCl
50 mM NaCl
1.0 mM DTT
0.1% mM EDTA
50% Glycerol
pH 7.5 @ 25°C
Supplied with:
10x Lambda Exo Reaction Buffer
10x Lambda Exo Reaction Buffer:
670 mM Glycine
25 mM MgCl2
500 μg/ml BSA
pH 9.4 @ 25°C
Unit Definition:
One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrates in 30 minutes at 37°C.
Recommended Storage Condition: -20°C
References:
1. Ausubel, F. M.,et al. (1987) Current Protocols in Molecular Biology (John Wiley and Sons, Inc.)
2. Little, J.W. (1981) Gene Amp. Anal., 2, 135-145
CRISPR-Cas systems are powerful tools for mediating nucleic acids. Cas12a (also referred as Cpf1) nuclease belonging to the Class 2, Type V CRISPR system.
PFS 5′-GAAAG-3′
Origin: Sulfuricurvum sp. PC08-66
Purity > 95%
Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. CasRx, Cas13d from Ruminococcus flavefaciens XPD3002, is highly active and short, 94KD.
Fast and simple fragmentation
Fragment size easily controlled by incubation time
Ideal for NGS library preparation
Fast and simple fragmentation
Fragment size easily controlled by incubation time
Ideal for NGS library preparation
Fast and simple fragmentation
Fragment size easily controlled by incubation time
Ideal for NGS library preparation
Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. Cas13a, (previously referred to as C2c2) is part of the Type VI CRISPR-Cas system.
Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic
repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. Cas13a, (previously referred to as C2c2) is part of the Type VI CRISPR-Cas system.
LE-100 5,000 units, 20,000 U/ml 120 €
LE-200 20,000 units 20,000 U/ml 361 €
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Čeština
Polski
