MC Lab (10)

The MCLAB's HoTaq™ SYBR Green One-Step RT-PCR Kit allows researchers to perform SYBR Green based RT-qPCR in a single step within a single tube.

5,000 U, 200 U/μl

ATP-dependent 3′ to 5′ helicase

expressed in E. coli

HEPES Solution is a biological buffer useful in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture, and degree of chemical definition required for the cell culture application.

• • activity at 45 °C to 70 °C
    purified from E. coli

• hydrolyzes the phosphodiester backbone at the 5' end of the AP site
• creates a gap

Sodium hydroxide is commonly used in the clandestine production of methamphetamine to change the pH of the solution to a basic pH so that it can become soluble in an organic solution.

5M solution of sodium chloride (NaCl) in high purity dH₂O. Solution is 0.2 μm filtered and dispensed into durable, square Nalgene bottles.

2,500 units, 10,000 units/ml

origin: E. coli carrying the Tn5 gene
random transposon insertion into DNA
requires 19-bp ME sequence

5,000 U, 20 U/μl

Kit do amplifikacji DNA RCA to nowatorski produkt opracowany specjalnie do przygotowywania matryc do sekwencjonowania DNA. Metoda RCA wykorzystuje polimerazę DNA bakteriofaga phi29 do eksponencjalnej amplifikacji jedno- lub dwuniciowych krążkowych matryc DNA poprzez amplifikację koła obrotowego (RCA). 

IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under the control of the lac operon.

Kanamycin Sulfate solution is an aminoglycoside antibiotic consisting of a six-membered aminocyclitol ring attached to aminosugar side chains through glycosidic bonds. Kanamycin Sulfate solution acts by inhibiting protein synthesis, and compromising translational fidelity in susceptible bacteria.

50,000 units, 200 units/μl

Ammonium acetate is a widely used reagent in molecular biology and chromatography. Suitable applications include the purification and precipitation of DNA and protein crystallization. Ammonium acetate is commonly used in HPLC and MS analysis of various compounds, such as oligosaccharides, proteins, and peptides.

Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation.

Ammonium Persulfate (APS) is an oxidizing agent that is used with TEMED to catalyze the polymerization of acrylamide and bisacrylamide to prepare polyacrylamide gels for electrophoresis.

MCLAB's RNA Storage Buffer, with optimized reagents and pH value, provides better preservation and greater RNA stability for valuable RNA samples than the standard EDTA and TE Buffer or just water.

Low concentration solutions of DNA are prone to DNA degradation or other loss. It is critical that optimal methods are employed for DNA suspension and long-term storage.