50% Triton X-114
TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C).
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Used to select for ampicillin resistance in mutated and transformed cells.
Description
Ampicillin sodium is a member of the extended spectrum β-lactam family and similar in structure to penicillin. Ampicillin resistance is due to cells containing plasmid encoded ESBLs. Ampicillin Sodium is freely soluble in water.
Ampicillin sodium targets non ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug." Resistance to ampicillin is routinely utilized as a selectable marker to confirm successful cell transformation.
Application
Used to select for ampicillin resistance in mutated and transformed cells.
Product Specifications
Sodium Ampicillin in H2O
(100 mg/ml Pre-Made Solution, 0.2µm Sterile Filtered)
Formula: C16H19N3O4S
Molecular Weight: 403.45
Recommended Storage Conditions: 2-8 °C
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TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C).
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Used to select for ampicillin resistance in mutated and transformed cells.
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